Standardisation of aseptic technique will assist in reducing hais and improve patient outcomes the terminology used clarifies, standardises and simplifies practice ensure your clinical practice is reflective of the new aseptic technique procedure. Standards unit, evaluations and standards laboratory page 1 of reference no. Aseptic techniques aseptic techniques also called sterile techniques are defined as the processes required for transferring a culture from one vessel to another without introducing any additional organisms to the culture or contaminating the environment with the culture. Produce nutrient agar na plates, na slants, and nutrient broths nbwhich will be used in. Refer to your laboratory text for various methods of transferring microbial cultures.
Practicing aseptic technique with microbial cultures flinn scientific. Aseptic technique is a crucial experimental condition in a microbiology lab. Aseptic technique and transfer of microorganisms biology. A pure culture is a type of culture that only contains one type of. Experiment 1 culture transfer technique purpose technique of aseptic removal and transfer of microorganisms for subculturing. This chapter describes common laboratory procedures that can reduce the risk of culture con taminations sepsis, collectively referred as aseptic technique. The simplest and most economical way to reduce contamination from airborne particles and aerosols e. Aseptic technique lab report growth medium experiment.
For the researcher new to cell culture, this handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation quality. This technique ensures a contaminant free environment while. Aseptic technique 2 introduction when working in a laboratory, aseptic technique is very crucial. The aseptic technique is one of a number of procedures that contributes to preventing health care associated infections hcai and is a means of preventing or minimising the risk of introducing harmful microorganisms into sterile areas of the body when undertaking clinical procedures doh 2008. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Aseptic technique is a means of performing lab work that greatly reduces the risk of contamination. Aseptic an environment or procedure that is free of contamination by pathogens bauman, robert w. Aseptic technique is a set of best practices that healthcare professionals use to prevent the transfer of germs in clinics and hospitals and protect patients from infection. Bacterial cells will settle to the bottom of a tube if sitting on ice or the bench for long periods of time. Previously, the terms sterile technique, clean technique and aseptic technique have been used interchangeably. Learn aseptic technique lab 2 with free interactive flashcards. Practicing aseptic technique with microbial cultures. It is in place to prevent contamination of bacterial cultures and the transfer of an infectious bacteria into its host. Materials and methods aseptic technique was used throughout the experiment.
This technique ensures a contaminant free environment while handling micro organisms. In order to illustrate that microorganisms are all around us and to demonstrate the necessity for proper aseptic technique, contaminate three trypticase soy agar plates as follows. Qsop 52i1 this sop should be used in conjunction with the series of other sops from the health protection agency uk email. The hypothesis for this experiment was that cultures inoculated with s. The purpose of the gram stain is to show whether the bacteria are gram positive purplecolored, gram negative pinkcolored, or both. Any unknown organism that you dont anticipate to culture on the agar plate is considered contamination. Media and equipment selection sterile work area for labs with cell culture hoods. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. The thioglycollate broth was used as a reducing agent that reacted with oxygen to form water, allowing for the determination of the oxygen requirements of the unknown lammert 2007. Aseptic technique microbiology resource center truckee. Microscopy and aseptic technique although robert hooke was the first person to publish work using a light microscope, antony van leeuwenhoek, is considered to have made a greater contribution to our understanding of the. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage.
The tube is initially filled with the medium and then collects gas as the bacteria grow, creating a. To demonstrate good aseptic technique in culture transfer or inoculation and in. The aseptic technique is a method of preventing the transmission of infection to the patient during the performance of various clinical procedures. Principles and practice of aseptic technique health care. To that aim, we have assembled this updated laboratory handbook of cell culture techniques. Preventing the contamination of a sample with foreign microbes from the environment is the main focus of the present experiment. Proper aseptic technique prevents contamination of cultures from. General aseptic techniques in microbiology laboratory basic. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests.
An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2 good microbiological laboratory practice gmlp 3 spillage management 3 aerosols 3 resources equipment 4 apparatus 5 materials 5 media, sterilisation and disinfection preparation of culture media 6 pouring a plate 6 storage of media 6. Preventing contamination of experimental materials with microbes from the environment, including from your body. Aseptic technique and culturing microbes lab 3 brittney case august 4th 20 section. Aseptic technique is imperative when working in a cell culture lab and the main effort should be done to provide a barrier between the microorganisms populating the environment and the uncontaminated cell culture. Aseptic technique lab report free download as word doc. Asepsis is the state of remaining free from pathogenic and contaminating microorganisms. Indications patients have a right to be protected from preventable. Remove the tube cap and hold it between your little finger and ring finger of the hand holding the loop see figure 2. Prevent contamination of the specific microorganism we are working with. The goal is to reach asepsis, which means an environment that is free of harmful microorganisms. Doc aseptic technique lab report mescopio aisyah academia. Media, incubation, and aseptic technique objectives 1. Durham tubes are used to detect the production of gases, such as co2 or n2, by microorganisms.
Aseptic technique steps, purpose and importance of aseptic. Aseptic techniques aseptic techniques are the precautionary measures taken to prevent contamination of pure cultures and sterile laboratory equipment. Contamination can also occur as a result of poor sterile technique or dirty lab conditions. Aseptic technique is absolutely necessary for the successful establishment and maintenance of plant cell, tissue and organ cultures. Briefly flame the lip of the tube while rotating the tube to ensure no microorganisms remain on the lip see figure 3. Describe the different formats of media, plate, tube etc. Remove the lid from the first agar plate and place the exposed agar portion in or out of the building for the duration of todays lab. General aseptic techniques in microbiology laboratory.
Plating methods article pdf available in journal of visualized experiments may 2012 with 16,993 reads how we measure reads. The following conditions must exist for aseptic technique to be successful. The third treatment was to use a nonsterile plate from a previous experiment while following all aseptic techniques to transfer stock bacteria. All materials coming in contact with the cells should be sterile and all the efforts should be done to maintain this sterility. Aseptic technique, designed to provide a barrier between the microorganisms in the environment and the sterile cell culture.
Cleaning and disinfecting lab surfaces prior to use. The elements of aseptic technique are a sterile work area, good personal hygiene, sterile reagents and media, and sterile. Aseptic technique good aseptic or sterile technique is of critical importance in many areas of biology, particularly in microbiology. Sterile aseptic technique michigan state university. Explain how to sterilize it, and how to distribute it in different formats. Microorganisms in the lab atmosphere may come to rest on the desktop between classes.
Aseptic technique is the range of infection prevention and control practices which are used to minimize the presence of pathogenic microorganisms during clinical procedures. Many of the organisms can be opportunistic in their abilities to cause infection. Pure culture techniques university of pennsylvania. A search for aseptic technique on the american society of microbiology website reveals that several lab experiments and job opportunities mention the importance of aseptic technique. Use aseptic technique throughout all manipulations of phage and bacteria. Aseptic technique is a procedure used by medical staff to prevent the spread of infection. The purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Choose from 500 different sets of aseptic technique lab 2 flashcards on quizlet. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. The correct practice of this technique requires the understanding of some principles and facts.
Biol 202each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit if the question asks you to do research or find a source, a reputable, credible andor scholarly source citation must be. It is a skill that must be used for all laboratory procedures when working with microorganisms. Reflection of the aseptic technique mercurial essays. Gram staining bacteria requires the use of aseptic technique to ensure the sterility of the experiment. Aseptic technique, designed to provide a barrier between the microorganisms in the environment and the sterile cell culture, depends upon a set of procedures to reduce the probability of contamination from these sources. Aseptic technique and the transfer of microorganisms. In the microbiology lab we use aseptic technique to. To minimize the risk of contamination, a method known as aseptic technique is practiced. Culturing and aseptic technique 100 points student name. Practicing aseptic technique with microbial cultures continued 2 21 linn cientiic nc all ights esere 6.
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